Peptidylglycine alpha-amidating monooxygenase mwa, contributing signatures
These areas also showed strong PAM-like immunoreactivity.
Interestingly, all pyramidal neurons of the hippocampus expressed very high levels of PAM mRNA, although no identified amidated peptide matches this distribution completely. The first step of the reaction is catalysed by peptidylglycine alpha-hydroxylating monooxygenase PHMand is dependent on copper, ascorbate and molecular oxygen; peptidyl-alpha-hydroxyglycine alpha-amidating lyase PAL catalyses the second step of the reaction [ PMID: To characterize the trafficking of integral membrane PAM proteins in neuroendocrine cells, we have generated stable AtT cell lines expressing full length and COOH-terminally truncated integral membrane PAM proteins .
The optimal pH for amidating activity was 5.
Much of the current information on this page has been automatically compiled from Pubmed. The expression of the gene encoding this enzyme was examined in adult rat brain by in situ hybridization histochemistry and immunocytochemistry.
Peptidylglycine alpha-amidating monooxygenase Peptidylglycine monooxygenase Enzyme Protein - others
WikiGenes is a non-profit and open access community project - Read more. The observed heterogeneous PAM mRNA levels may reflect differences in the peptidergic activity of different neuronal systems.
PAM mRNA levels were found to be 3-fold higher in prostate adenocarcinomas compared with that of human benign prostate hyperplasia BPH as demonstrated by real-time quantitative reverse transcription-PCR . PAM transcripts could be localized in certain ventricular ependymal cells, with the highest expression in the lateral ventricle.
PHM is required for normal developmental transitions and for biosynthesis of secretory peptides in Drosophila. Alternative splicing governs sulfation of tyrosine or oligosaccharide on peptidylglycine alpha-amidating monooxygenase .
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Ideally this entry shall become one comprehensive and continuous article. The protein contains two enzymes, peptidylglycine alpha-hydroxylating monooxygenase PHM and peptidyl-alpha-hydroxyglycine alpha-amidating lyase PALthat act sequentially to catalyse the alpha-amidation of neuroendocrine peptides [ PMID: There have been significant advances in the understanding of the dioxygen -activation chemistry of mononuclear copper monooxygenases such as peptidylglycine alpha-amidating monooxygenase and dopamine beta-monooxygenase DbetaM .
A comparison of PAM activity and DDC levels in 30 lung cancer cell lines indicated that peptide amidating activity may be an indicator of NE status . The copper-enzyme family of dopamine beta-monooxygenase and peptidylglycine alpha-hydroxylating monooxygenase: